Plasmid_Backbone

Part:BBa_J433115

Designed by: Asimov   Group: Asimov   (2023-06-26)


Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin

Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin from Asimov Mammalian Collection

Clone in with BsaI GCTT and TACA Fusion Sites

Clone out into L2 with SapI AGG and ACC Fusion Sites

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 1776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2050
    Illegal SapI.rc site found at 5


[edit]
Categories
Parameters
None